人源低密度脂蛋白 (Human LDL 
货号：YB-001 ) 氧化型低密度脂蛋白 (Human Ox-LDL, OxLDL 货号：YB-002 ) 小鼠低密度脂蛋白 (Mouse LDL 货号：YB-006 ) 小鼠氧化低密度脂蛋白 (Mouse Ox-LDL 货号：YB-008 ) 牛源低密度脂蛋白 (Bovine LDL 货号：YB-0015 ) 牛源氧化低密度脂蛋白 (Bovine Ox-LDL 货号：YB-0016 ) 高氧化程度低密度脂蛋白 (Human High Ox-LDL, High OxLDL 货号：YB-002-1) 乙酰化低密度脂蛋白 (Human Ac-LDL, AcLDL 货号：YB-004) DiI标记氧化型低密度脂蛋白 (Human DiI-Ox-LDL 货号：YB-0010) DiI标记乙酰化低密度脂蛋白 (Human DiI-Ac-LDL 货号：YB-0013) DiI标记低密度脂蛋白 (Human DiI-LDL 货号：YB-0011) 高密度脂蛋白 (Human HDL 货号：YB-003) DiI标记高密度脂蛋白 (Human DiI-HDL 货号：YB-0017) 极低密度脂蛋白 (Human VLDL 货号：YB-009) DiI标记极低密度脂蛋白 (Human DiI-VLDL 货号：YB-0014) 无脂蛋白血清 (Human Lipoprotein-free Serum or Lipoprotein Deficient Serum 货号：YB-005) 抗人SR-A抗体(Anti-SR-A)，抗CD36抗体(Anti-CD36)，抗人Ox-LDL抗体，抗人ApoE抗体奕元提供以下技术服务:
① 分离低密度脂蛋白
② 分离高密度脂蛋白
③ 分离极低密度脂蛋白
④ 任意密度蛋白质超速离心分离
备注:
客户提供各类实验动物血浆或其它类型样本（通过冷冻/冷藏快递给我司），我们提供55000转（超过20万g）以上的超速离心服务，透析，超滤浓缩技术服务，帮客户分离纯化:
① 低密度脂蛋白
② 高密度脂蛋白
③ 极低密度脂蛋白
④ 按客户要求的任意密度的蛋白分离
LDL is a large protein (MW 3,500 kDa) with a diameter of 25.8 nm.It is composed of approximately 20-25% protein and 75-80% lipid. The lipid portion can be further described as 9% free cholesterol, 42% cholesteryl ester, 20-24% phospholipid, and 5% triglyceride.
Our LDL is membrane filtered and aseptically packaged. Store @ 4oC.
Our Human LDL is oxidized using Cu2SO4 (oxidant) in PBS. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0fold further than the native LDL.
Yiyuanbiotech Native-LDL(n-LDL), Acetylated-LDL(Ac-LDL) and Oxidized-LDL(ox-LDL) were loaded on a agarose gel and electrophoresed for 60 minutes. The lipoproteins were stained with Sudan Black(A and B). Oil red O stainning was used to determine the formation of foam cell. RAW264.7 were incubated with with 80μg/mL ox-LDL for 24hrs.
Mouse LDL is membrane filtered and aseptically packaged under nitrogen in a solution containing phosphate-buffered saline at pH 7.4 and 0.2mM EDTA-Na2.
Mouse Oxidized LDL is membrane filtered and aseptically packaged under nitrogen in a solution containing phosphate-buffered saline at pH 7.4 and 0.1mM EDTA-Na2 (PBS+EDTA-Na2 Cat. No. YB-101 is used as vehicle control).
Bovine LDL is membrane filtered and aseptically packaged under nitrogen in a solution containing phosphate-buffered saline at pH 7.4 and 0.2mM EDTA-Na2.
Bovine Oxidized LDL is membrane filtered and aseptically packaged under nitrogen in a solution containing phosphate-buffered saline at pH 7.4 and 0.1mM EDTA-Na2 (PBS+EDTA-Na2 Cat. No. YB-101 is used as vehicle control).
Human LDL is oxidized extensively oxidized with Cu2SO4 (oxidant) in PBS at 37°C. It is used to induce cell apoptosis/death and cell injure. More detail about this product is available by sending a e-mail to: yiyuanbiotech@126.com
LDL is acetylated with acetic anhydride and dialyzed. It is ultrafiltered through a membrane and packaged aseptically under nitrogen.
oxidized Low Density Lipoprotein, labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate, which is highly fluorescent lipophilic dyes that diffuse into the hydrophobic portion of the LDL complex without affecting the LDL-specific binding of the apoprotein. DiI-Ox-LDL is used to label both vascular endothelial cells and macrophages. It can be used to identify and/or isolate these cells from mixed cell populations and investigate uptake and binding of modified LDL by different cell types. DiI has been excited at 488nm or 514nm and the emission is detected at 565 nm.
Representive confocal images of suptake of DiI-ox-LDL in macrop-
hages from ApoE knockout mice.
Acetylated Low Density Lipoprotein, labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate(see DiI-Ox-LDL ). Modified low-density lipoprotein (LDL) including Ox-LDL, Ac-LDL can bind to cell membrance via class A scavenger receptor (SR-A) , CD36 and LOX-1 etc. When cells are labeled with DiI-Ac-LDL, the lipoprotein is degraded by lysosomal enzymes and the DiI (fluorescent probe) accumulates in the intracellular membranes. It can be used to quantify uptake of modified LDL. It also be used to identify isolated cells including vascular endothelial cells, macrophages, and endothelial progenitor cells(EPC).
Confocal images indicate
binding of DiI-Ac-LDL in
macrophages membrance
Low Density Lipoprotein labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate(see DiI-Ox-LDL ).
Human High Density Lipoprotein, HDL, (Cat. No. YB-003) is isolated from blood bank produced human plasma. It is purified via ultracentrifugation (d=1.063 ～1.21 g/mL) to homogeneity determined by agarose gel electrophoresis.
VLDL (d= <1.006 g/mL) is isolated from human plasma by ultracentrifugation. The purified VLDL is ultrafiltered through a membrane and packaged aseptically under nitrogen.
Purified YB VLDL is labeled with the fluorescent probe, DiI, and reisolated by ultracentrifugation (d <1.006 g/mL). The resultant product is exhaustively dialyzed against phosphate buffered saline, (pH 7.4), sterilized by membrane filtration and then aseptically packaged in a solution containing phosphate-buffered saline at pH 7.4 and 0.1 mM EDTA-Na2.
The macrophage class A scavenger receptor (SR-A) and CD36 mediate the uptake of modified low-density lipoprotein (LDL) including Ox-LDL, Ac-LDL, anti-SR-A and anti-CD36 is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of SR-A of human origin.
Western blot analysis of SR-A
expression in THP-1 whole cell
lysate
